Glossary

sgRNA

Single-guide RNA used to target a gene in CRISPR-KO, CRISPRi, or CRISPRa screens.

pegRNA

Prime-editing guide RNA. Different pegRNAs can share a spacer but differ in RTT or PBS, so spacer alone is not a unique identity.

Variant

A defined genomic REF-to-ALT change. Prime Editing Build requires GRCh38 coordinates and checks the REF allele.

MOI

Mean effective viral integrations per cell in the Poisson planning model. At MOI 0.3, approximately 85.7% of infected cells are expected to carry one integration.

Representation

Number of cells, independent DNA templates, or sequencing reads per library reagent. Always check the unit and experimental stage.

Biological replicate

Independently conducted infection, culture, treatment, and harvest. Resequencing the same sample is a technical replicate.

T0

Baseline sample collected before the experimental endpoint comparison.

gDNA

Genomic DNA extracted from cells, containing integrated guide cassettes for abundance sequencing.

Gini coefficient

Summary of count inequality across a plasmid library; zero denotes perfectly equal counts.

Log2 fold change

Log2 ratio of normalized endpoint and baseline abundance for a reagent.

FDR

False discovery rate reported for a specified statistical analysis and contrast. It does not by itself validate a biological mechanism.

PRIDICT2

External pegRNA candidate-prediction engine; its score is not a measured editing fraction.

pegLIT

External engine for epegRNA linker engineering.

MAGeCK

External statistical software used for screen abundance analysis.

See Documentation for planning assumptions and References for external software and library sources.