Review modality

Keep the biological target consistent from planning through review. Prime Editing MAVE compares pegRNA and variant representation; gene-level projects compare sgRNA and gene representation.

Planned versus observed

Enter planning values for the current gene-level project, then record observed sgRNA and gene QC outputs.

Interpretation

Use Review after QC and Analyze to identify representation losses before interpreting gene-level biological effects. A detected reagent count is not a cellular representation measurement.